Beneficial Effects of Omega 3 and Vitamin E Co-Administration on Gene Expression of SIRT1 and PGC1α and serum antioxidant enzymes in patients with Coronary Artery Disease

Esmaeil Yousefi Rad, Somayeh Saboori, Fariba Koohdani, Ebrahim Nematipour, Ali Akbar Saboor-Yaraghi, Mohammad Hassan Javanbakht, Mohammad Reza Eshraghian, Atena Ramezan, Mahmoud Djalali


AbstractBackground and Aim: SIRT1 and PGC1α are two important genes which play critical rolesin regulating oxidative stress and inflammation processes. The aim of this study was to assessthe effects of omega 3 and vitamin E supplement co-administration on SIRT1 and PGC1αgene expression and serum levels of antioxidant enzymes in CAD patients.Methods and Results: Participants of this randomized controlled trial included 60 CADmale patients who received omega 3 (4g/day) and vitamin E placebo (OP), omega 3 (4g/day)and vitamin E (400IU/day) (OE) or omega 3 and vitamin E placebos (PP) for two months.Gene expression of SIRT1 and PGC1α in Peripheral Blood Mononuclear Cells (PBMCS) wasassessed using RT-PCR. Furthermore, serum antioxidant enzymes and hsCRP were assessedat the beginning and the end of the intervention. Gene expression of SIRT1 and PGC1α increased significantly in OE group (P =0.039 and P= 0.050, respectively). Catalase andhsCRP increased significantly in OE and OP groups. GPX and SOD did not statisticallychange in all groups. TAC increased significantly in OE group (P=0.009), but not in OP andPP groups.Conclusion: Combination of omega 3 fatty acids with vitamin E may have beneficial effectson CAD patients by increasing gene expression of SIRT1 and PGC1α and improvingoxidative stress and inflammation in these patients.Keywords: CAD, omega 3, vitamin E, SIRT1, PGC1α, antioxidant enzymes

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